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vesicular stomatitis virus g vsvg envelope expressing plasmid pmd2 g  (Addgene inc)


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    Addgene inc vesicular stomatitis virus g vsvg envelope expressing plasmid pmd2 g
    Vesicular Stomatitis Virus G Vsvg Envelope Expressing Plasmid Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vsvg+envelope+expressing+plasmid+pmd2%2Eg/pS2513%C2%B7PHP+(Plasmid+%23122590)/bio_rxiv__2025__08__07__669196-171-7-16
    Average 94 stars, based on 32 article reviews
    vesicular stomatitis virus g vsvg envelope expressing plasmid pmd2 g - by Bioz Stars, 2026-09
    94/100 stars

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    Produced:

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells.
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [14] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Article Title: A novel iPSC model reveals selective vulnerability of neurons in multiple sulfatase deficiency.
    Article Snippet: .. NGN2-iN lentiviruses were produced by polyethylenimine (PEI, Polysciences, 23966–2) in HEK 293 T cells (ATCC, VA) using 3rd generation lentiviral packaging plasmids pMDLg/pRRE (Addgene Inc., Plasmid 12251) and pRSV-Rev (Addgene Inc., Plasmid 12253), the VSVG envelope expressing plasmid (Addgene Inc., Plasmid 12259), in addition to the plasmid pLVX-UbC-rtTA-Ngn2:2A:EGFP (Addgene Inc., Plasmid 127288). .. Plasmids were removed after 8 h, and HEK 293 T cells were replenished with mTeSR Plus medium (StemCell Technologies).

    Article Title: Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families reveals complex genetic architecture and phenocopies.
    Article Snippet: Q5 site-directed mutagenesis (NEB) was performed on the PMS2-pCW57.1 plasmid to generate PMS2 variants of interest. .. PMS2-pCW57.1 constructs were packed into lentiviral particles produced by transient transfection of 293T cells using PolyJet transfection reagent as directed by the manufacturer (SignaGen Laboratories) using a 2.5:2.5:1 ratio of pCW57.1 plasmid, psPAX2 packaging vector, and pMD2.G VSVg envelope expression vector (Addgene). ..

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [ ] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Transfection:

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells.
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [14] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Article Title: Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation
    Article Snippet: .. For transfection in a 12-well, 0.1 μg vesicular stomatitis virus G (VSVG) envelope expressing plasmid pMD2.G (Addgene 12259), 0.4 μg lentiviral packaging vector psPAX2 (Addgene 12260), 1 μg lentiviral vector, and 3 μL JetPrime DNA transfection reagent were mixed with 75 μL JetPrime buffer (VWR, 89129924), incubated at room temperature for 10 minutes, and added drop wise to 293T cells. ..

    Article Title: Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families reveals complex genetic architecture and phenocopies.
    Article Snippet: Q5 site-directed mutagenesis (NEB) was performed on the PMS2-pCW57.1 plasmid to generate PMS2 variants of interest. .. PMS2-pCW57.1 constructs were packed into lentiviral particles produced by transient transfection of 293T cells using PolyJet transfection reagent as directed by the manufacturer (SignaGen Laboratories) using a 2.5:2.5:1 ratio of pCW57.1 plasmid, psPAX2 packaging vector, and pMD2.G VSVg envelope expression vector (Addgene). ..

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [ ] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Construct:

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells.
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [14] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Article Title: Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families reveals complex genetic architecture and phenocopies.
    Article Snippet: Q5 site-directed mutagenesis (NEB) was performed on the PMS2-pCW57.1 plasmid to generate PMS2 variants of interest. .. PMS2-pCW57.1 constructs were packed into lentiviral particles produced by transient transfection of 293T cells using PolyJet transfection reagent as directed by the manufacturer (SignaGen Laboratories) using a 2.5:2.5:1 ratio of pCW57.1 plasmid, psPAX2 packaging vector, and pMD2.G VSVg envelope expression vector (Addgene). ..

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [ ] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Expressing:

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells.
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [14] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer.
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressingGFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to themanufacturer’s instructions.

    Article Title: Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation
    Article Snippet: .. For transfection in a 12-well, 0.1 μg vesicular stomatitis virus G (VSVG) envelope expressing plasmid pMD2.G (Addgene 12259), 0.4 μg lentiviral packaging vector psPAX2 (Addgene 12260), 1 μg lentiviral vector, and 3 μL JetPrime DNA transfection reagent were mixed with 75 μL JetPrime buffer (VWR, 89129924), incubated at room temperature for 10 minutes, and added drop wise to 293T cells. ..

    Article Title: A novel iPSC model reveals selective vulnerability of neurons in multiple sulfatase deficiency.
    Article Snippet: .. NGN2-iN lentiviruses were produced by polyethylenimine (PEI, Polysciences, 23966–2) in HEK 293 T cells (ATCC, VA) using 3rd generation lentiviral packaging plasmids pMDLg/pRRE (Addgene Inc., Plasmid 12251) and pRSV-Rev (Addgene Inc., Plasmid 12253), the VSVG envelope expressing plasmid (Addgene Inc., Plasmid 12259), in addition to the plasmid pLVX-UbC-rtTA-Ngn2:2A:EGFP (Addgene Inc., Plasmid 127288). .. Plasmids were removed after 8 h, and HEK 293 T cells were replenished with mTeSR Plus medium (StemCell Technologies).

    Article Title: Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families reveals complex genetic architecture and phenocopies.
    Article Snippet: Q5 site-directed mutagenesis (NEB) was performed on the PMS2-pCW57.1 plasmid to generate PMS2 variants of interest. .. PMS2-pCW57.1 constructs were packed into lentiviral particles produced by transient transfection of 293T cells using PolyJet transfection reagent as directed by the manufacturer (SignaGen Laboratories) using a 2.5:2.5:1 ratio of pCW57.1 plasmid, psPAX2 packaging vector, and pMD2.G VSVg envelope expression vector (Addgene). ..

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressing GFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to the manufacturer’s instructions.

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [ ] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Plasmid Preparation:

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells.
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [14] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer.
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressingGFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to themanufacturer’s instructions.

    Article Title: Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation
    Article Snippet: .. For transfection in a 12-well, 0.1 μg vesicular stomatitis virus G (VSVG) envelope expressing plasmid pMD2.G (Addgene 12259), 0.4 μg lentiviral packaging vector psPAX2 (Addgene 12260), 1 μg lentiviral vector, and 3 μL JetPrime DNA transfection reagent were mixed with 75 μL JetPrime buffer (VWR, 89129924), incubated at room temperature for 10 minutes, and added drop wise to 293T cells. ..

    Article Title: A novel iPSC model reveals selective vulnerability of neurons in multiple sulfatase deficiency.
    Article Snippet: .. NGN2-iN lentiviruses were produced by polyethylenimine (PEI, Polysciences, 23966–2) in HEK 293 T cells (ATCC, VA) using 3rd generation lentiviral packaging plasmids pMDLg/pRRE (Addgene Inc., Plasmid 12251) and pRSV-Rev (Addgene Inc., Plasmid 12253), the VSVG envelope expressing plasmid (Addgene Inc., Plasmid 12259), in addition to the plasmid pLVX-UbC-rtTA-Ngn2:2A:EGFP (Addgene Inc., Plasmid 127288). .. Plasmids were removed after 8 h, and HEK 293 T cells were replenished with mTeSR Plus medium (StemCell Technologies).

    Article Title: Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families reveals complex genetic architecture and phenocopies.
    Article Snippet: Q5 site-directed mutagenesis (NEB) was performed on the PMS2-pCW57.1 plasmid to generate PMS2 variants of interest. .. PMS2-pCW57.1 constructs were packed into lentiviral particles produced by transient transfection of 293T cells using PolyJet transfection reagent as directed by the manufacturer (SignaGen Laboratories) using a 2.5:2.5:1 ratio of pCW57.1 plasmid, psPAX2 packaging vector, and pMD2.G VSVg envelope expression vector (Addgene). ..

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressing GFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to the manufacturer’s instructions.

    Article Title: Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACE hi Cells
    Article Snippet: The pCG1-Spike-HA construct encoding the Wuhan SARS-CoV-2 Spike Envelope glycoprotein was kindly provided by Dr Stefan Pölmann [ ] and the pCMV3-2019-nCoV-Spike(S1+S2)-long Flag and untagged (VG40589-CF and VG40589-UT) were purchased from Sino Biological Europe GmbH (Eschborn, Germany). .. Lentivector particles were produced by calcium phosphate DNA transfection of HEK293T cells with the HIV-1 packaging construct pSPAX2, the miniviral genome bearing the expression cassette encoding GFP (FUGW-GFP), and the plasmid encoding either the SARS-CoV-2 Spike Envelope glycoproteins or the VSVg envelope expressing plasmid pMD2.G (addgene). ..

    Luciferase:

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer.
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressingGFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to themanufacturer’s instructions.

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressing GFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to the manufacturer’s instructions.

    Generated:

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer.
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressingGFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to themanufacturer’s instructions.

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressing GFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to the manufacturer’s instructions.

    Stable Transfection:

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer.
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressingGFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to themanufacturer’s instructions.

    Article Title: Altered ribosomal profile in acquired resistance and reversal associates with pathological response to chemotherapy in inflammatory breast cancer
    Article Snippet: .. SUM149, rSUM149, and rrSUM149 cells expressing green fluorescent protein and Luciferase dual reporters were generated by stably transducing with lentivirus expressing GFP using the pBMN(CMV-copGFP-Luc2-Puro plasmid (Addgene plasmid # 80389) and co-transfected with pMD2.G VSVG envelope expressing plasmid (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), both second-generation lentiviral packaging plasmids. .. Briefly, 293T cells were transfected with constructs using jetPRIME® Packaging Mix (Polyplus, NY, USA) for lentiviruses production, according to the manufacturer’s instructions.

    Virus:

    Article Title: Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation
    Article Snippet: .. For transfection in a 12-well, 0.1 μg vesicular stomatitis virus G (VSVG) envelope expressing plasmid pMD2.G (Addgene 12259), 0.4 μg lentiviral packaging vector psPAX2 (Addgene 12260), 1 μg lentiviral vector, and 3 μL JetPrime DNA transfection reagent were mixed with 75 μL JetPrime buffer (VWR, 89129924), incubated at room temperature for 10 minutes, and added drop wise to 293T cells. ..

    Incubation:

    Article Title: Perturb-seq reveals distinct responses to pluripotency regulator dosages underlying the control of self-renewal and differentiation
    Article Snippet: .. For transfection in a 12-well, 0.1 μg vesicular stomatitis virus G (VSVG) envelope expressing plasmid pMD2.G (Addgene 12259), 0.4 μg lentiviral packaging vector psPAX2 (Addgene 12260), 1 μg lentiviral vector, and 3 μL JetPrime DNA transfection reagent were mixed with 75 μL JetPrime buffer (VWR, 89129924), incubated at room temperature for 10 minutes, and added drop wise to 293T cells. ..



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